Table S1 - Restriction Enzyme Body Doubles and PCR Cloning: On the General Use of Type IIS Restriction Enzymes for Cloning

Summarizing list of the used PCR primers, ENases, vectors and reaction conditions. Primers: Digested overhangs by the BD enzymes are marked by CAPITAL LETTERS, the recognition sites of the BD enzymes are marked by italic. We generally use BsmBI as a BD enzyme as it cuts very close to its recognition site (which results in relatively short primers). Annealing temperature: two different degrees are shown when the temperature was raised after five cycles. Number of cycles: the temperature was raised after five consecutive cycles when needed. The pSB plasmid was a kind gift of Z. Ivics and Zs. Izsvák [30]. The pACS-KDEL-SSIL2-SBP-EGFP-GPI plasmid was a kind gift of F. Perez [31]. His: histidine (6×, 10×), hMGFP: monster green fluorescent protein, inz: inzulator, mPrP: mouse Prion protein (wt: wild type), EYFP: enhanced yellow fluorescent protein (ML: first metionin is mutated to leucin to avoid the expression of a signal peptide-less protein, Nyeste and Welker, unpublished results), cys: cysteine, GPI: signal sequence of the glycophosphatidylinositol phosphate anchor, SS: ER-targeting signal sequence (the signal sequences usually comes from the protein whose core sequence is incorporated in the plasmid construct, otherwise it is marked), mSho: mouse Shadoo protein, IL2: interleukine-2, KDEL: endoplasmic reticulum retention signal, SBP: streptavidin binding peptide (PDF)