Julien Mouysset, Alexandra Deichsel, Sandra Moser, Carsten Hoege, Anthony A. Hyman, Anton Gartner, Thorsten Hoppe (2011) CIL:25628, Caenorhabditis elegans, early embryonic cell. CIL. Dataset

This movie illustrates the early division up to the four-cell stage of a C. elegans embryo expressing YFP::CDC-48 by DIC and fluorescence microscopy. YFP::CDC-48, like YFP::UFD-1 and YFP::NPL-4, are cytosolic during mitosis, but accumulate in the nucleus after its reformation. The first C. elegans embryonic division of the P0 zygote is asymmetric and generates an anterior AB cell, and a smaller posterior P1 cell. These cells have different developmental fates and division timing, with AB dividing approximately 2 min before P1. Additionally, in early C. elegans embryonic cell cycles, S and M phases rapidly alternate without apparent gap phases. The movie corresponds to experiments shown in Fig. 1A and Fig. S1 and Movie 1 of Mouysset et al. Movie is CIL 25628 and original data file is CIL 35123.

本影片展示了秀丽隐杆线虫胚胎在表达YFP::CDC-48蛋白的情况下，通过DIC和荧光显微镜观察到的早期分裂直至四细胞期的过程。YFP::CDC-48，与YFP::UFD-1和YFP::NPL-4相似，在细胞分裂过程中位于细胞质中，但在细胞核重组后积累于其中。P0受精卵的第一个秀丽隐杆线虫胚胎分裂是不对称的，生成一个前端的AB细胞和一个较小的后端P1细胞。这些细胞具有不同的发育命运和分裂时间，AB细胞分裂时间大约早于P1细胞2分钟。此外，在秀丽隐杆线虫早期胚胎细胞周期中，S期和M期快速交替，并无明显的间期。该影片对应于Mouysset等人在图1A、图S1和电影1中展示的实验，电影编号为CIL 25628，原始数据文件编号为CIL 35123。