A truncated reverse transcriptase enhances prime editing by split AAV vectors

To investigate if the truncated PE can be dilivered by dual AAV8 vectors for in vivo prime editing. We injected the dual AAV8 into 10-week-old C57BL/6J mice . Livers were isolated 4 weeks after injection and next generation sequencing showed an average of 1.4% and 5.4% precise prime editing with the low and high AAV doses, respectively (Figure 4D ). This demonstrates that PECO-Mini can be efficiently delivered by dual AAVs for in vivo prime editing. Overall design: Dual AAV8 were injected into 10-week-old C57BL/6J mice intravenously at an equal vector amount of either 1x1011 or 1x1012 vg per vector. The mice without any treatment was used as control. We injected 3 mice for each type with a total of 9 mice.