Transcriptome profiling of haloperidol treated mouse bone marrow LSK cells

We performed RNA sequencing analyses of adult mouse bone marrow lineage-negative, Sca-1-positive, and c-kit-positive (LSK) hematopoietic stem/progenitor cell population. Especially, we investigated gene expression profiling of LSK cells before and after haloperidol treatment. Overall design: Wildtype(B6) mice were injected with DMSO(vehicle) or Haloperidol for consecutive 3 days. Then the LSK cells were sorted by FACS. During FACS sorting, cells were still exposed to DMSO or haloperidol treatment. FACS sorted LSK cells were directly collected in RLT lysis buffer and processed for RNA-Seq.