Systematic Identification and Analysis of Lysine Succinylation in Strawberry Stigmata

Strawberry is an important economic crop in the world, and strawberry stigmata are vital to promote pollen germination and allow pollen tubes to enter into stigma tissue. Post-translational modification (PTM) of proteins can significantly affect protein structure and function, and plays a very important regulatory role in plant development. So the functional study of PTM during the pollen-stigma recognition process will improve understanding of the molecular mechanism of the pollen-stigma recognition reactions in strawberry plants. However, to date, little is known about the functions of PTM in strawberry stigmata. In this study, we first analyzed various protein PTMs that likely exist in strawberry stigmata, and found lysine succinylation is highly abundant among six types of PTM. Then a highly efficient enrichment method for succinylation peptides was used to identify 209 succinylation modification sites in 120 proteins in the stigmata.