Targeting CAFs-Mediated Stromal Signaling in a Patient-Derived Organotypic Colorectal Tumor Model

The goal of this experiment is to understand why co-culturing colon cancer cells with carcinoma associated fibroblasts (CAFs) causes the colon cancer cells to develop chemo-resistance. We cultured a patient-derived colon cancer cell model derived from conditional reprogramming with or without carcinoma-associated fibroblasts. Before sequencing, the co-cultured cells were purified using EpCAM immuno-magnetic beads to isolate cancer cells. Overall design: There are duplicate samples of colon cancer cultured alone or with colon cancer cells isolated after co-culture with fibroblasts.