five

Polyadenylation site mapping of xrn1? in Sacchromyces cerevisiae

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https://www.ncbi.nlm.nih.gov/sra/SRP285324
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The use of alternative polyadenylation sites is common and affects the post-transcriptional fate of mRNA, including its stability, localization, and translation. Here we use the internal version of our previously developed protocol (PMID: 23295673), to characterize the polyA sites in a xrn1? strain. Overall design: PolyA tail mapping at high resolution of a XRN1 deletion strain, with two biological replicates.
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2020-09-27
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