Additional file 1 Table S1. Mutation list of CRC panel. Table S2. Primer sequences for mutation detection by CRC panel. Table S3. Primer sequences for mutation validation by Sanger sequencing. (XLS 14
Probe-based fluorescence melting curve analysis (FMCA) is a powerful tool for mutation detection based on melting temperature generated by thermal denaturation of the probe-target hybrid. Nevertheless
A novel multiple mutation technology that integrates nucleotide enrichment (NE)-assisted specific identification of variant alleles with matrix-assisted laser desorption/ionization time-of-flight mass
*mutation detected only by HRM and sequencing; by microarray analysis, the sample was identified as wild-type since no specific reporter was designed for the mutation.†mutation detected by COLD-PCR an
