Organ Mapping Antibody Panel (OMAP) for Multiplexed Antibody-Based Imaging of Human Skin with Cell DIVE, v1.1

OMAP-22 was developed for Cell DIVE multiplexed imaging of human formalin-fixed, paraffin-embedded (FFPE) skin tissue.<br>Cell DIVE enables highly multiplexed, antibody-based imaging and single-cell analysis, as outlined in Gerdes et al.<br>(2013). Tissue slides were processed with a dual antigen retrieval method using pH 6 and pH 9 buffers, as described in<br>McDonough et al. (2020), and antigens were detected with either dye-conjugated primary antibodies or unlabeled<br>primaries with fluorophore-conjugated secondaries. A detailed protocol is available in Pryhuber et al. (2022). The<br>panel comprises 34 antibodies and the nuclear marker DAPI for image registration and nuclear segmentation. This<br>updated v1.1 OMAP builds on OMAP-4 (McDonough, Chadwick, and Ginty 2024) and expands biomarker coverage by including<br>CD103, CD14, CD1a, CD207, CD34, F13A1, CDKN1A, KRT1, KRT5, KRT7, KRT10, MLANA, PDPN, TPSAB1, and VWF. These additions<br>enable identification of specific keratinocyte populations, dermal dendritic cells, melanocytes, mast cells, and<br>Langerhans cells. Protein biomarkers such as DDB2, CDKN1A, and p53 allow profiling of aging and skin damage responses.<br>Updates also include the use of commercially available conjugates when possible, replacing custom-labeled clones, and<br>correction of CD3 marker specification from CD3D to CD3E. This OMAP provides spatial context for most anatomical<br>structures and cell types listed in the ASCT+B skin table v1.3 (Ginty et al. 2023). Representative datasets are<br>publicly available on the HuBMAP data portal.<br><br>**Bibliography:**<br><br>* Gerdes, Michael J., Christopher J. Sevinsky, Anup Sood, Sudeshna Adak, Musodiq O. Bello, Alexander Bordwell, Ali Can, et al. 2013. “Highly Multiplexed Single-Cell Analysis of Formalin-Fixed, Paraffin-Embedded Cancer Tissue.” *Proceedings of the National Academy of Sciences* 110 (29): 11982–87. https://doi.org/10.1073/pnas.1300136110.<br>* Ginty, Fiona, Jonhan Ho, Joel Sunshine, and Arivarasan Karunamurthy. 2023. “Skin (v1.3) Graph Data, v1.3.” 2023. https://lod.humanatlas.io/asct-b/skin/v1.3/.<br>* McDonough, Elizabeth, Chrystal Chadwick, and Fiona Ginty. 2024. “4-Skin-Cell-Dive (v1.3) Graph Data, v1.3.” 2024. https://lod.humanatlas.io/omap/4-skin-cell-dive/v1.3/.<br>* McDonough, Liz, Chrystal Chadwick, Fiona Ginty, Christine Surrette, and Anup Sood. 2020. “Cell DIVETM Platform | Slide Clearing and Antigen Retrieval,” December. https://www.protocols.io/view/cell-dive-platform-slide-clearing-and-antigen-retr-bpwumpew.<br>* Pryhuber, Gloria, Gail Deutsch, Christine Surrette, Lisa Lowery, Heidie Huyck, and Cory Poole. 2022. “HuBMAP | GE/URMC/SCH Cell DIVETM Modality Overview,” February. https://www.protocols.io/view/hubmap-ge-urmc-sch-cell-dive-modality-overview-b4kgqutw.