Cloning of Human ABCB11 Gene in E. coli required the removal of an Intragenic Pribnow-Schaller Box before it’s Insertion into Genomic Safe Harbor AAVS1 Site using CRISPR Cas9

Sequencing data and original images including some of the failed experiments can be found here. Context: Please refer the manuscript. Sequencing data: 1) Cloning of ABCB11--sequencing 2) Cloning of gRNA to two Cas9 vectors 3) Sequencing for ABCB11 integration to HEK293T cell line genome at AAVS1 site 4) Sequencing for off target sites-AAVS1 site HEK293T cell genomic DNA PAGE and Western Blot Data: 1) E. Coli lysate transformed ad untransformed DH5a PAGE and W/B with anti ABCB11 antibody 2) ABCB11 transfected cell lines--whole protein fibroblasts 3) ABCB11 integration mediated by Cas9 cell line-whole protein fibroblasts 4) Puromycin selection cellines HEK Agaraose gel images: T7 assay and PCRs Comet Assay