Next Generation Sequencing Quantitative Analysis of soybean seeds Transcriptomes

To investigate the regulatory mechanisms of GmZF392, GmZF351 and GmNFYA in oil accumulation, seeds at H3 stage of transgenic plants and JACK plants were collected for RNA extraction and sequencing. Before sequencing, qPCR showed that expression of GmZF392, GmZF351 and GmNFYA increased to about 10, 109 and 7 times respectively compared to JACK. Clean reads of RNA-sequencing were mapped to soybean genome and genes that had a 1.5 fold increase or 50% decrease, compared to JACK, were defined as differential expressed genes (DEGs). Overall design: mRNA profiles of soybean seeds at H3 stage. Wild type (Jack), GmZF351 overexpressing soybean, GmZF392 overexpressing soybean and GmNFYA overexpressing soybean.