Asymmetric reproductive interference of male Drosophila suzukii caused by both male and female Drosophila melanogaster

Reproductive interference, leading to negative effects on fitness, usually occurs within two closely related species which sharing overlapping ecological niches. Our preliminary experiments showed D. suzukii population decreased when reared with D. melanogaster. However, the explanation of this phenomenon is not much clear. Based on the overlapping ecological niches and courtship behaviours of D. suzukii and D. melanogaster, we deduced that reproductive interference was the dominant reason. We found that the proportion of heterospecific orientation time is significantly greater in male D. suzukii (7.5 ± 4.183 %) than in male D. melanogaster (4.439 ± 4.936 %, U=194.5, Z=2.223, P=0.026). The successful mating rate of D. suzukii was significantly lower when the species was reared with D. melanogaster (F1,31=4.303, p=0.046). The egg production of D. suzukii was significantly lower in the combined rearing condition (F1,343=98.623, p<0.001), for D. melanogaster, the egg production in combi...,

Male conspecific and heterospecific courting behaviours

To ensure that the flies used for courting observations were unmated, we placed each pupa into 2-ml Eppendorf tubes for emergence. All tubes were maintained in incubators with a light:dark photoperiod of 14:10 h, temperature of 25±1°C, and humidity of 60±5%. All flies were kept in Eppendorf tubes for 48 h from eclosion until observation started. By comparing the male courtship behaviours of D. suzukii and D. melanogaster (Revadi et al. 2015; Spieth 1974), we selected seven specific behaviours—orientation, tapping, wing scissoring and spreading, circling, successful mating, heterospecific mating attempts and premating duration—for observation (Table 2). Proportion of misdirected courting time to total courting time in the two files were also recorded. Based on the rearing conditions, D. suzukii and D. melanogaster were divided into three groups (Figure 1): (1) D. suzukii single rearing (DSS): two pairs of D. suzukii; (2) D. ..., , # Asymmetric reproductive interference of male Drosophila suzukii caused by both male and female Drosophila melanogaster

[https://doi.org/10.5061/dryad.bzkh189gn](https://doi.org/10.5061/dryad.bzkh189gn)

## Description of the data and file structure

80 vedios and a supplementary materia file about reproductive interference between *Drosophila suzukii* and *Drosophila melanogaster* are included.

### Files and variables

File: DSS-1.avi

Description: D. suzukii single rearing group 1

File: DSS-2.avi

Description: D. suzukii single rearing group 2

File: DSS-3.avi

Description: D. suzukii single rearing group 3

File: DSS-4.avi

Description: D. suzukii single rearing group 4

File: DSS-5.avi

Description: D. suzukii single rearing group 5

File: DSS-6.avi

Description: D. suzukii single rearing group 6

File: DSS-7.avi

Description: D. suzukii single rearing group 7

File: DSS-8.avi

Description: D. suzukii single rearing group 8

File: DSS-9.avi

Description: D. suzukii single rearin...,