Pacific oyster physiology data with different microalgal diets 2020-2022

72 adult Pacific oysters collected from Woolston bay in Southampton in October 2021 and fed one of three microalgal diets: Isochrysis galbana, Nannochloropsis gaditana/oculata, or an equal mix of Isochrysis and Nannochloropsis. Data include total weight, shell weight, internal tissue weight, respiration rate, and expression levels of four shell-forming genes for all oysters. Stable isotope (SI) composition of the Isochrysis galbana and Nannochloropsis gaditana were measured, as was the SI of the stomach of oysters fed the mixed species diet. 72 Pacific oysters were fed either brown alga Isochrysis galbana, green alga Nannochloropsis gaditana/oculata, or an equal cell density of both, for 11 weeks. The effect of these diets on oyster physiology was measured using: respirometry to calculate respiration rate, cell clearance and rejection rate by counting algal cell density and pseudofaeces before and after feeds, weight gain (both of the shell and the internal tissues), comparative stable isotopic analysis of the microalgae and the oyster guts, and the expression of four shell-forming genes. Temperature, pH, ammonia, nitrite, nitrate, and dissolved oxygen in the seawater was also measured.