Co-expression network analysis of frontal cortex during the progression of Alzheimer’s disease

Using WGCNA and enrichment analyses to identify pathway level differences between individuals with no cognitive impairment, mild cognitive impairment, and Alzheimer’s disease. Frozen frontal cortex (BA10) tissue from NCI, MCI, and mild/moderate AD cases (n = 12/group) representing both genders was acquired postmortem from participants in the Rush Religious Orders Study, a longitudinal clinical pathologic study of aging and AD in elderly Catholic clergy Nimblegen expression array human frontal cortex - NCI (No Cognative Impairment) vs. MCI (Mild Cognative Impairment) vs. AD (alzheimers disease); Labeled cDNA was digested and hybridized to NimbleGen 12 x 135K human arrays for 18 hrs at 42°C and analyzed on a GenePix 4200A scanner (Molecular Devices). Probe intensity levels were quantified with RMA preprocessing (NimbleScan v2.5, Roche)