Transcriptomic studies of CrHAM KD gametophytes in Ceratopteris richardii

This study aims to comprehensively determine the downstream signaling of CrHAM in controlling gametophyte development in Ceratopteris. High-throughput RNA sequencing (RNA-seq) was performed on developing hermaphrodites and males from the same wild-type (WT) or CrHAM KD population at 5 days after germination (DAG). To determine transcriptomic changes in CrHAM KD hermaphrodites at a late developmental stage without the presence of exogenous antheridiogen, WT and CrHAM KD hermaphrodites were individually cultured and collected at 8 DAG for the RNA-seq experiment.