Braf-mutant Schwann cells divert to a repair phenotype to induce congenital demyelinating neuropathy [ScN]

RASopathies are a diverse group of developmental disorders associated with germline or somatic mutations in genes of the Mitogen Activated Protein Kinase (MAPK) signaling pathway. We characterized in this dataset a mosaic mouse model, in which a constitutively active form of the MAPK effector Braf (V600E) is expressed in the embryonic progenitors of myelin-forming Schwann cells of peripheral nerves under the control of the MpZ(P0)-Cre recombinase. These mice develop an early, fully penetrant degenerative peripheral neuropathy. The RNAseq experiment compares total RNA extracted from whole sciatic nerves between spinal cord and knee between mutant and control pairs of littermates from six distinct outbred litters at postnatal day 21. We performed RNA-seq to compare four mutant whole sciatic nerves to four control littermate sciatic nerves at two different postnatal stages. After aligning sequences to the mouse genome, we undertook differential gene expression analysis.