Immune-mediated Effects of Lactitol in Animals with Antibiotic-induced Dysbiosis after Infection with Clostridium Difficile

The research objective was to study the effect of dysbiosis in mice caused by the treatment with combination of amoxicillin trihydrate and potassium clavulanate on the colonization with Clostridium difficile (C. difficile) and course of C. difficile infection (CDI), to estimate the effect of the prebiotic lactitol when was administered with antibiotic for prevention and therapy of the CDI. Antibiotic-induced dysbiosis was modeled with solution of Amoxicillin and Clavulanate that was administered into mice i/g twice a day for 10 days with a dosing interval of at least 8 hours. A single dose of Am and Cl was 585 mg/kg and 83.62 mg/kg, respectively. Lactitol was administered i/g twice a day. A single dose of lactitol was 1740 mg/kg. Mice received lactitol in a prophylactic regimen; it was given starting from the first day of antibiotic administration and then for 21 days. Fecal samples of the animals were taken on 10th and 21th days of the study in each group.