Deficiency of ciliary Wdr60 and Wdr34 cause distinct embryonic neural malformation phenotypes

Cilia are specialized organelles that extend from plasma membrane, functioning as antennas for signal transduction of multiple pathways involved in embryonic morphogenesis. Heterodimer WDR60-WDR34 are intermediate chains of motor protein Dynein-2, playing vital roles in ciliary retrograde transport. To explore functions of WDR60 and WDR34 in neurodevelopmental signaling pathways, we leveraged piggyBac (PB) transposon to establish Wdr60 and Wdr34 mouse mutants separately and gathered E10.5 Wdr60 PB/PB and Wdr34 PB/PB tissues for RNAseq experiments. Each experimental or control group included three biological replicates.