LSU Bioinformatics Workshop course materials

RNA sequencing (RNA-Seq) is a powerful tool that captures information
about how organisms respond to stimuli in their environment at the
molecular level. A common RNA-Seq approach involves isolating and
sequencing all of the messenger RNA (mRNA) in a tissue sample taken from
an organism. Researchers can compare patterns observed in RNA-Seq data to
understand how individuals respond to the environment over minutes, hours,
or days and how populations evolve in response to the environment over
millions of years. The materials in this repository will guide
users through an analysis of RNA-Seq data collected from two California
populations of a copepod crustacean, Tigriopus californicus, that were
exposed to different levels of salinity. Users will examine the contents
of a fastq file that contains raw RNA-Seq data, determine the quality of
the RNA-Seq data using a web-server, and test for significant differences
in gene expression between the copepod populations using the R packages
DESeq2, edgeR, and VennDiagram. The course materials were developed for
the 2022 LSU Bioinformatics Workshop held at Louisiana State University,
Baton Rouge, LA on July 17-22, hosted by Melissa DeBiasse and Morgan
Kelly.