RNAseq expression libraries from Mycobacterium abscessus with deletion of the sRNA B11

WT M. abscessus ATCC_19977 was used to construct a targeted deletion mutant lacking the sRNA B11 and a complemented version of the mutant with ectopic expression of B11 from its native promoter at the L5 site. The deletion mutant was also complemented with versions of B11 that lacked a single C from the first loop or had an extra C in the second loop. All strains were grown to log phase and the extracted RNA was used to perform expression profiling by RNAseq. Overall design: Triplicate cultures were grown to mid-log phase in 7H9 supplemented with glycerol, Tween 80, and ADC. RNA was extracted from each cultured, subject to rRNA depletion, and used to contruct Illumina RNAseq libraries.