Mosaic genome of the Mollicutes-related endobacteria of the arbuscular mycorrhiza fungus Dentiscutata heterogama. Mollicutes-related endobacteria of Dentiscutata heterogama

In this study, we sequenced the genome of a Mollicutes-related endobacteria (MRE) living in the arbuscular mycorrhiza fungus Dentiscutata heterogama, termed DhMRE. To study the genomic organization and gene repertoire of DhMRE we isolated the DNA of the endobacterial population from spores of D. heterogama FL654. Following Illumina sequencing and PCR and Sanger sequencing based gap-closing approaches, three DhMRE genomic scaffolds could be defined. One is circular, with a size of 648,879 bp and 34.1% GC content (scaffold A), the second one 124 (scaffold B) is 60,375 bp in size with a GC content of 32.3% and the third (scaffold C) is a 125 putative plasmid of 3,779 bp and 34.1% GC content. The total size of the DhMRE genome draft assembly is 713,033 bp, with an estimated sequence coverage of ca. 130-fold.