Anopheles funestus FUMOZ rp1 (resistance to pyrethroid 1) BAC sequence. Anopheles funestus FUMOZ rp1 (resistance to pyrethroid 1) BAC sequence

Analysis described in Wondji et al. (2009). A BAC library derived from the mosquito Anopheles funestus (FUMOZ colony) was produced at The Institute for Genomic Research (TIGR). After screening to identify the cytochrome P450 genes CYP6P9 and CYP6P4, a single BAC clone was grown overnight at 37°C, harvested in glycerol solution and stored at −80°C. The BAC clone was fragmented by sonication into 2-5kb fragments that were cloned into plasmid vectors (pC31) and sequenced using an ABI DNA Sequencer. Shotgun sequence reads were trimmed and vector sequence removed using Seqman, then assembled using phrap.Wondji, C.S., Irving, H., Morgan, J., Lobo, N.F., Collins, F.H., Hunt, R.H., Coetzee, M., Hemingway, J. and Ranson, H. (2009) Two duplicated P450 genes are associated with pyrethroid resistance in Anopheles funestus, a major malaria vector. Genome Research 19(3). 452-459.