Metabolic incorporation of 14C-oleic acid-derived radioactivity by Mtb wild-type and d-facl6 mutant.
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Mtb wild-type and d-facl6 mutant in log-phase or subjected to dormancy-inducing conditions were incubated with radiolabel as described in Materials and Methods. Radioactivity (DPM, disintegrations per minute) in total lipid extracts was determined by counting dried aliquots by scintillation counting. Radioactivity in triacylglycerol (TAG) and polar lipids (PL) was determined by liquid scintillation counting after TLC separation of total lipid extracts.Metabolic incorporation of 14C-oleic acid-derived radioactivity by Mtb wild-type and d-facl6 mutant.
创建时间：
2015-12-02
