metabolomics of zebrafish at different temperatures

Temperature has a significant impact on the feeding, reproduction, and metabolism of organisms. The metabolome refers to the endogenous small molecules that make up the metabolic components in an organism's cells, tissues, and organs. The complex metabolic activities and their regulation in an organism play a crucial role in its growth, development, immunity, and other aspects. Metabolites, which are the downstream end-products of gene and protein expression, serve as endpoints for bioinformation transmission and can provide a comprehensive reflection of the organism's physiological and pathological state. In this study, we conducted zebrafish culturing experiments at different water temperatures (HT, 28 ℃/LT, 16 ℃) and employed high-throughput liquid chromatography-mass spectrometry (LC/MS) to investigate the metabolic profiles of the zebrafish gut. The results revealed significant differences in metabolites at different temperatures, and these differences were strongly associated with changes in gut microbes.