SRCAP Mutations Drive Clonal Hematopoiesis Through Epigenetic and DNA Repair Dysregulation [ATAC-Seq]

To investigate how Srcap mutations confer mutant hematopoietic stem and progenitor cells (HSPCs) with selective advantage after transplantation We assessed chromatin remodeling and chromatin accessbility before and after transplantation to understand the mechanism of the acquisition of Srcap selective advantage We established Srcap heterozygous mutant murine competitive transplantation model. Donor test (CD45.2+) HSPCs in the bone marrow were sored and isolated at 12 weeks post-transplantation for ATAC-seq