Brain–muscle tissue communication prevents muscle aging by maintaining daily physiology

A network of molecular clocks is crucial for coordinating daily physiology and maintaining organismal health. However, the mechanisms underlying the interactions between these clocks and the significance of intra-tissue clock networks in muscle tissue maintenance have remained elusive. To uncover this network structure, we established a minimal clock module with the central clock (suprachiasmatic nucleus/brain) and/or a peripheral clock (muscle) in arrhythmic mice with premature aging. We find that reconstituting the brain-muscle clock network alone is sufficient to preserve fundamental daily homeostatic functions and prevent premature muscle aging. However, achieving whole muscle daily physiology requires the contribution of other peripheral clocks. Mechanistically, the muscle peripheral clock acts as a gatekeeper, selectively suppressing signals from the central clock that could be detrimental to muscle function if left uncontrolled while also integrating important muscle homeostatic ..., , , # Brain–muscle tissue communication prevents muscle aging by maintaining daily physiology

[https://doi.org/10.5061/dryad.8931zcrxp](https://doi.org/10.5061/dryad.8931zcrxp)

## Data

Each Excel file contains a sheet with a list of relevant variables and their definitions.

### Supplementary tables S1 to S4 legends:

Table S1. Rhythmic transcriptome output by JTK_CYCLE in defined mice genotypes, with 12:12 hour light/dark (LD) cycles.

Table S2. Rhythmic transcriptome output by RAIN in defined mice genotypes, with 12:12 hour LD cycles.

Table S3. Differential rhythmicity analysis dryR in defined mice genotypes, with 12:12 hour LD cycles.

Table S4. Transcription factor motif enrichment analysis using g:Profiler, with 12:12 hour LD cycles.