Real-time quantitative PCR analysis of induced pluripotent derived endothelial cells. Real-time quantitative PCR analysis of induced pluripotent derived endothelial cells

CD31+/VECad+ hiPSC-ECs were purified through magnetic activated cell sorting. Expanded endothelial cells were then subjected to serum starvation conditions for 24 hours by culturing in DMEM. After serum starvation, total RNA was extracted. We used a custom Thermo Fisher Scientific PCR assay panel to quantify gene expression relevant to ciliogenesis, cytoskeletal integrity, Wnt signaling, pluripotency and endothelial phenotype. Overall design: Biological Replicates: BC1-ECs (n=3); C12-ECs (n=2); hiPSC-ECs (n=3). Technical triplicates were performed for each gene analyzed.