Sequence of transgenic insert incorporating reporter genes GFP-LacZ-E2F-RFP within HEK293 cells. HEK293 GFP-LacZ-E2F-RFP

HEK293 cells over expressing GFP-LacZ with bicistronic (E2F) RFP. Little data about the transgene was supplied; consequently primers were designed and used to amplify contiguous regions spanning the transgene. This was done to identify the specific sequence expressed to allow the creation of siRNA and antisense oligonucleotides necessary to turn of the expression of this transgender. This was necessary to evaluate the efficiency of specific intracellular drug delivery technologies. In addition sequencing allowed the identification of the specific GFP, LacZ and RFP variants expressed. Comparison of in silico translated transgenic sequence identified the sequence as coding fro emerald GFP, E.coli LacZ chain 1 and the RFP tandem dimeric tomato separated from the EmGFP-LacZ sequence by an E2F bicistronic spacer.