A BRD4-mediated elongation control point primes transcribing RNA polymerase II for 3'-processing and termination [ChIP-seq]

In this study, we reveal that BRD4 underlies a general 5'-elongation checkpoint that primes transcribing RNA polymerase II for 3'-RNA processing and transcription termination. BRD4-specific degradation impairs Pol II pause release, induces massive readthrough transcription, and RNA cleavage defects. Acute loss of BRD4 disrupts the recruitment of 3'-RNA processing factors. Overall design: ChIP-Rx measurements for Pol II, Pol II Ser2-P, SPT5, PAF1, FIP1, CPSF73 and CstF64 upon DMSO (control) and dTAG7 (BRD4 degradation) treatment in K562 dTAG-BRD4 cells (two biological replicate measurements and matched input controls for each condition).