Data from: PLGA‑encapsulated cross‑linked GALC aggregates as a hierarchical nanostructured platform for enzyme stabilization and delivery

This dataset contains raw experimental data supporting the research paper: Colagiorgio, L., Gagliardi, M., Grosso, A. D., Carpi, S., Sarlo, M. D., & Cecchini, M. (2026). PLGA‐Encapsulated Cross‐Linked GALC Aggregates as a Hierarchical Nanostructured Platform for Enzyme Stabilization and Delivery. International Journal of Biomaterials, 2026(1), 8346433, doi: 10.1155/ijbm/8346433.The collection includes physicochemical measurements, protein assays, and in vitro biological evaluation data used to characterize and validate the nanostructured platform. Structure and File Contents Physicochemical and Biochemical Characterization 'CLEAs screening size': Raw Dynamic Light Scattering (DLS) data for size characterization during the screening phase of Cross-Linked Enzyme Aggregates (CLEAs) (Figure 3a-b,d-f). 'CLEAs screening protein assay': Raw microplate reader spectrophotometric data for protein quantification during CLEAs screening (Figure 3c). 'CLEAs scale-up size': Raw DLS data regarding size distribution during the optimization of the CLEAs scale-up process (Figure 4a-b,d-f). 'CLEAs scale-up enzyme activity': Raw microplate reader spectrophotometric data for protein quantification during CLEAs scale-up process (Figure 4c). 'NP-CLEAs optimization size': Raw DLS data regarding particle size optimization of PLGA nanoparticles encapsulating CLEAs (NP-CLEAs) (Figure 5a-b,d-f). 'NP-CLEAs optimization enzyme activity': Raw microplate reader spectrophotometric data for enzyme activity during optimization of PLGA nanoparticles encapsulating CLEAs (NP-CLEAs) (Figure 5c). 'Stability Ctrl - 4 °C' and 'Stability pe-CLEAs - 4 °C': Raw DLS data regarding size distribution during stability tests at 4 °C (Figure 6a). 'Stability Ctrl - 37 °C' and 'Stability pe-CLEAs - 37 °C': Raw DLS data regarding size distribution during stability tests at 37 °C (Figure 6b). 'Stability Ctrl -Enzyme activity - 4 °C' and 'Stability pe-CLEAs - 4 °C': Raw microplate reader spectrophotometric data for enzyme activity during stability tests at 4 °C (Figure 6c). 'Stability Ctrl -Enzyme activity - 37 °C' and 'Stability pe-CLEAs - 4 °C': Raw microplate reader spectrophotometric data for enzyme activity during stability tests at 37 °C (Figure 6d). In Vitro Biological and Functional Assays 'HOM cell viability.pzf': Raw cell viability assay data conducted on a homozygous cell line (Figure 7a). 'WT cell viability.pzf': Raw cell viability assay data conducted on a wild-type cell line (Figure 7a). 'ERT in vitro.pzf': Raw data evaluating functional efficacy for in vitro Enzyme Replacement Therapy (ERT) (Figure 7b). Data Formats and Software Requirements Files with the '.pzf' extension are native project files created in GraphPad Prism. They contain replicate data tables, associated statistical analyses, and the layouts of the figures presented in the article. Files containing DLS and microplate reader measurements are provided in open tabular formats, accessible via standard data analysis tools (e.g., Microsoft Excel, or equivalent OpenSource softwares). Compressed folders are in .zip format and can be opened with several free softwares (e.g. 7-zip).