De novo RNA sequencing of the gut and cerebral ganglion of Dendrobaena veneta exposed to carbamazepine

One of the most widely used drugs in municipal wastewater treatment effluents and soil is carbamazepine, a commonly prescribed antidepressants and antiepileptic drug. Carbamazepine exerts an intrinsic biological activity on the nervous system, thus may induce ecotoxicological effects on non-target organisms. Earthworms, one of the essential indicator species of soil health, accumulate biosolid fertilisers and wastewater contaminants. In this project, earthworms (Dendrobaena veneta) were treated with carbamazepine to explore their uptake dynamics, molecular and life cycle endpoints. By conducting transcriptomic profiling of different tissues in an organism exposed to carbamazepine assists in defining detoxification and neural system responses in the terrestrial invertebrate. Overall design: De novo RNA sequencing (RNASeq) was carried out via the HiSeq X Ten platform to define the transcriptomic fingerprints in the gut and cerebral ganglion tissue of earthworms exposed to 0.6 or 10 mg/kg carbamazepine added to the topsoil under laboratory conditions for 28 days.