Listeria monocytogenes infected mouse liver RNA sequencing

IFN-Is have a detrimental effect in the case of Listeria monocyteogenes (Lm) infection. Previously it has been shown that Ifnar-/- mice are protected from the infection induced death. However, we have found that IRF9, a downstream component of the IFNAR signalling, has a role in addition to the IFNAR signalling in protecting the mice from Lm infection. This is due to Irf9-/- mice being more protected than Ifnar-/- mice. Among other hypotheses, we think that the recently shown role of Irf9 in glucose metabolism can be effective in this additional protection. Furthermore, our metabolic screens point to similar direction. Therefore, we would like to measure the differential gene expression pattern in livers from uninfected and 1 day infected Wt, Irf9-/- and Ifnar-/- mice. RNA isolated from the livers of biological triplicates of wt, Irf9-/-, Ifnar-/- mice kept uninfected and infected with Lm for 1 day