Supplemental Material: Long-term metabolic and developmental effects of in vitro embryo technologies in female cattle

Supplementary Table 1A. Body weight (kg) of animals born by artificial insemination (AI) and embryo transfer with frozen, fresh or vitrified embryos measured monthly until 15 months of age.

Supplementary Table 1B. circumference (cm) of animals born by artificial insemination (AI) and embryo transfer with frozen, fresh or vitrified embryos measured monthly until 15 months of age.

Supplementary Table 1C. Chest circumference (cm) of animals born by artificial insemination (AI) and embryo transfer with frozen, fresh or vitrified embryos measured monthly until 15 months of age.

Supplementary Table 2. Samples and animals used in the analysis of methylation and gene expression of IGF2 (samples from individuals older than 30 days were grouped into arbitrary age periods).

Supplementary Table 3A. IGF2 expression in blood lymphocytes from animals at different ages (time points).

Supplementary Table 3B. IGF2 methylation levels in blood lymphocytes from animals at different ages (time points).

Supplementary Table 4. Calves born from embryos cultured in the different culture medium (CM) corresponding to Table 3. From day-0 to day-6, embryos were cultured in groups, and from day-6 to day-7, embryos were cultured individually.

Supplementary Table 5. Probability for the squared Mahalanobis distance exposed in Table 7 between ART (i.e., artificial insemination or embryo transfer –ET– with fresh or cryopreserved embryos) in the Day-0 and Day-7 plasma metabolomes.

Supplementary Table 6. Probability values of effects used to build the models of metabolite concentrations that differed on Day-0 in blood plasma of adult females born from different reproductive technologies, i.e., artificial insemination (AI) and embryo transfer (ET) with fresh, frozen and vitrified embryos.

Supplementary Table 7. Probability values of effects used to build the models of metabolite concentrations that differed on Day-7 in blood plasma of adult females born from different reproductive technologies, i.e., artificial insemination (AI) and embryo transfer (ET) with fresh, frozen and vitrified embryos.

Supplementary Table 8. Concentrations (mg/L) of metabolites affected by culture conditions from Day-6 to Day-7 in single drops of SOF containing BSA, HDGF-PVA or PVA vs. females born from artificial insemination (AI), as measured on Day-0 and Day-7 blood plasma of the adult female offspring resulting from such embryos.