RhoJ controls EMT associated resistance to chemotherapy

To identify potential mechanisms by which EMT tumour cells are resistant to chemotherapy, we assessed the expression of putative mediators of resistance to therapy in Epcam+ and Epcam- tumour cells from skin squamous cell carcinoma. To determine how RhoJ impacts the transcriptional landscape of Epcam- tumour cells, we performed RNA seq of sh-RNA RhoJ knock-down Epcam- tumour cells. Comparative gene expression profiling analysis of RNA-seq data for FACS isolated Epcam+ or Epcam- YFP+ tumour cells from Lgr5CreER/KRasG12D/p53cKO/ Rosa-YFP mice; Comparative gene expression profiling analysis of RNA-seq data for Epcam- cells grown in culture and transduced with RhoJ sh-RNA or control sh-RNA. 2 cell lines were studied in each condition.