Porcine cell-free system mass spectrometry compiled data sets

The degradation of sperm-borne mitochondria after fertilization is a
conserved event. This process known as post-fertilization sperm mitophagy,
ensures exclusively maternal inheritance of the mitochondria-harbored
mitochondrial DNA genome. This mitochondrial degradation is in part
carried out by the ubiquitin proteasome system. In mammals,
ubiquitin-binding pro-autophagic receptors such as SQSTM1 and GABARAP have
also been shown to contribute to sperm mitophagy. These systems work in
concert to ensure the timely degradation of the sperm-borne mitochondria
after fertilization. We hypothesize that other receptors, cofactors, and
substrates are involved in post-fertilization mitophagy. Mass spectrometry
was used in conjunction with a porcine cell-free system to identify other
autophagic cofactors involved in post-fertilization sperm mitophagy. This
porcine cell-free system is able to recapitulate early fertilization
proteomic interactions.  Altogether, 185 proteins were identified
as statistically different between control and cell-free treated
spermatozoa. Six of these proteins were further investigated, including
MVP, PSMG2, PSMA3, FUNDC2, SAMM50, and BAG5. These proteins were
phenotyped using porcine in vitro fertilization, cell imaging, proteomics,
and the porcine cell-free system. The present data confirms the
involvement of known mitophagy determinants in the regulation of
mitochondrial inheritance and provides a master list of candidate
mitophagy co-factors to validate in the future hypothesis-driven studies.