Human Protein Atlas - Subcellular

The subcellular resource of the Human Protein Atlas provides high-resolution insights into the expression and spatiotemporal distribution of proteins encoded by 13534 genes (67% of the human protein-coding genes), as well as predictions for an additional 3491 secreted- or membrane proteins, covering a total of 17025 genes (84 % of the human protein-coding genes). For each gene, the subcellular distribution of the protein has been investigated by immunofluorescence (ICC-IF) and confocal microscopy in up to three different standard cell lines, selected from a panel of 41 cell lines used in the subcellular resource. For some genes, the protein has also been stained in up to three ciliated cell lines and/or in human sperm cells. Upon image analysis, the subcellular localization of the protein has been classified into one or more of 49 different organelles and subcellular structures. In addition, the resource includes an annotation of genes that display single-cell variation in protein expression levels and/or subcellular distribution, as well as an extended analysis of cell cycle dependency of such variations.
The subcellular resource offers a database for detailed exploration of individual genes and proteins of interest, as well as for systematic analysis of proteomes in a broader context. More information about the content of the resouce, as well as the generation and analysis of the data, can be found in the Methods summary.
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The subcellular distribution of proteins in human cell lines.
The subcellular distribution of proteins in human sperm.
The proteomes of different organelles and subcellular structures.
Single-cell variability in the expression levels and/or localizations of proteins.

人类蛋白质图谱提供的亚细胞资源，以高分辨率展现了13534个基因（占人类蛋白质编码基因的67%）编码蛋白的表达及其时空分布情况，并对另外3491个分泌蛋白或膜蛋白进行了预测，涵盖了总计17025个基因（占人类蛋白质编码基因的84%）。对于每个基因，其编码蛋白的亚细胞分布情况均通过免疫荧光（ICC-IF）和共聚焦显微镜在最多三种标准细胞系中进行研究，这些细胞系均来自41个细胞系面板，用于亚细胞资源的研究。对于某些基因，其蛋白还可在最多三种纤毛细胞系以及/或人类精子细胞中进行染色。经过图像分析，蛋白的亚细胞定位被归类为49种不同的细胞器和亚细胞结构之一或多个。此外，该资源还包括了对在蛋白表达水平及/或亚细胞分布上表现出单细胞变异的基因的注释，以及对这些变异的细胞周期依赖性的扩展分析。该亚细胞资源为对特定基因和蛋白进行详细探索以及在对更广泛背景下的蛋白质组进行系统分析提供了一个数据库。关于资源内容、数据生成和分析的更多信息，可在方法总结中查阅。了解以下内容：
人类细胞系中蛋白的亚细胞分布。
人类精子中蛋白的亚细胞分布。
不同细胞器和亚细胞结构的蛋白质组。
蛋白表达水平和/或定位的单细胞变异。