File S1 - Sharing of Potential Nest Sites by Etheostoma olmstedi Males Suggests Mutual Tolerance in an Alloparental Species

Contains supporting figures, tables, and text. Figure S1: Schematic of the tank set-up used in this study. Pictured is an A) top-down, and B) side view of the tank set-up used. All objects are scaled to their correct relative sizes. Tanks were 31.1 H×41.3 W×59.7 L (cm). AS = air stone, ST = small tile (10×10 cm), LT = large tile (14×28 cm). The curved line in picture B indicates water height. Table S1: Comparisons of sharing by females of different size ranks. Same-sex sharing between females of specific size ranks was not influenced by the density condition. Tests are one-way ANOVAs or unpaired t-tests (test statistic indicated). Sample sizes for each density condition were: four-fish, N = 21; six-fish, N = 20; eight-fish, N = 21 (N = 20 for comparison of largest and smallest females due to missing size data; see methods). Table S2: Comparisons of preferred position in the tank. The random chance of using a location was considered as the percentage of the total involved area (therefore, tiles represents 19.9% of the total tank area, and the large tile represents 79.7% of the “under tile” area in a tank). Binomial tests considering both methods of determining “random” placement were run. All p-values are two-tailed. a) Females were more likely than expected by chance (based on the area of the tank covered by tiles) to spend a majority of checks under the tiles rather than out. b) Females use of the large tile rather than the small tile did not differ from the usage expected by chance (based on the relative surface area of the tiles). (DOCX)