Transcript and metabolic analysis during the accumulation of Bletilla striata polyacrylamide

In transcriptome analysis, a total of 6767 DEGs were identified, and differentially expressed genes in the S1-VS-S2 period were significantly more than those in the S2-VS-S3 period. In the 40 entries of GO enrichment analysis, these genes were mainly enriched in biological processes in the three stages. Most DEGs are classified as secondary metabolism, cellular processes, biological regulation, multicellular organism processes and emergency response. KEGG enrichment analysis mainly involved 160 pathways, of which 122 differential genes were enriched in glucose metabolic pathways such as starch and sucrose metabolism, galactose metabolism, pentose phosphate pathway, fructose and mannose metabolism, and amino sugar and nucleotide sugar metabolism.