Mapping the response of human osteocytes in native matrix to mechanical loading using RNA sequencing. Mapping the response of human osteocytes in native matrix to mechanical loading using RNA sequencing

Aim: To identify differentially expressed genes by mapping the response of human primary osteocytes in their native matrix to mechanical loading using RNA sequencing. Overall design: Comparative gene expression profiling analysis of RNA-seq data of osteocytes in human cortical bone, which was not loaded or mechanically loaded by 2000 or 8000 µɛ for 5 min, followed by 0, 6, or 24 h post-culture without loading.