Global gene expression analysis of human embryonic stem cells, adult fibroblasts, and CD34+ cord blood (CB) cells before, during, and after generation of stromal primed and non-stromal primed episomal induction of pluripotency

Global gene expression analysis of (a) human embryonic stem cells, (b) adult fibroblasts with and without nucleofection of SOKM, (c) CD34+ cord blood cells at various time points during induction of pluripotency with SOKM, with or without co-culture with bone marrow stromal cells (BMSC), and (d) resulting stromal primed and non-stromal primed cord blood CD34+ myeloid iPSC Total RNA was harvested from adult fibroblasts, adult fibroblasts three days after nucleofection with 4F (SOKM), human myeloid progenitors: Non-nucloefected CD34+ Day -3 unstimulated cord blood (CB) cells, Day 0 growth factor stimulated CB cells, Day +3 CB cells with or without nucleofection at Day zero and with or without Day zero to Day +3 co-culture with bone marrow stromal cells (BMSC), followed by FACS-purification of CD45+ myeloid progenitors, Day 23 CB cells nucleofected on day zero and co-cultured on BMSC from Day Zero to Day +3, undifferentiated H9 human embryonic stem cells, and low passage episomal stromal-primed iPSC derived from growth-factor activated CD34+ myeloid progenitors from cord blood or GCSF mobilized peripheral blood. Illumina HumanHT-12 V4.0 expression beadchips were used for all samples in this series. Three independent samples of each condition were each run on individual microarrays.