Clarke, Margaret, Silverman, Philip (2010) CIL:7323, Escherichia coli, prokaryotic cell. CIL. Dataset

This is one of four related images of F-pili (conjugative pili encoded by the F plasmid of E. coli) dynamics by live-cell imaging. There are 2 movie (.avi) files and 2 files (.tiff) from the movies. The movies show that F-pili normally undergo cycles of extension and retraction in the absence of any obvious triggering event such as contact with a recipient cell.

Retraction of an F-pilus. E. coli strain HfrH expressing the red fluorescent marker DsRed Express were mixed with R17 bacteriophage labeled with Alexa 488 (green); R17 bacteriophage (NCBITaxon:12026) bind to the sides of F-pili.

Samples were imaged by using a Zeiss LSM 510 confocal microscope with a Plan-Apochromat 63 × 1.4 N.A. DIC objective. Alexa-488 was excited with the 488-nm line of an argon laser with a 505–530-nm filter for emission, and DsRed was excited with the 543-nm line of a HeNe laser, with a 560-nm long pass filter for emission. An HFT UV/488/543 beam splitter was used. Images were collected in a single focal plane at 2 s intervals and accompanying movies (.avi) are playing at 10fps.

本数据集包含四幅相互关联的F-菌毛（由大肠杆菌F质粒编码的接合性菌毛）动态图像。其中包含两个电影文件（.avi格式）和两个由电影生成的文件（.tiff格式）。电影展示了F-菌毛在没有任何明显触发事件（如与受体细胞的接触）的情况下，通常会经历伸长和收缩的周期性变化。F-菌毛的收缩过程。表达红色荧光标记DsRed Express的大肠杆菌菌株HfrH与标记有Alexa 488（绿色）的R17噬菌体混合；R17噬菌体（NCBITaxon:12026）绑定在F-菌毛的侧面。样品使用蔡司LSM 510共聚焦显微镜，配备63倍1.4数值孔径的Plan-Apochromat DIC物镜进行成像。Alexa-488激发使用氩激光的488纳米波长，并通过505-530纳米的滤光片进行发射，DsRed激发使用氦氖激光的543纳米波长，并通过560纳米的长通滤光片进行发射。使用HFT UV/488/543分束器。图像以每2秒一次的间隔在单个焦平面上收集，并伴随播放的影片（.avi格式）以每秒10帧的速度进行。