The circadian regulator PER1 promotes cell reprogramming by inhibiting inflammatory signaling from macrophages

Fibroblasts can be reprogrammed to induced neurons (iNs) via transducion of Brn2, Ascl1, and Myt1l (BAM). To understand the roles of the circadain regulators Per1 and Per2 on the reprogramming, embryonic fibroblasts from wild type (WT), Per1-/-, and Per2-/- mice were transduced with the three genes and the cells were harvested on day 0, 2, and 4 after transduction for RNA-seq. Overall design: Embryonic fibroblasts from wild type (WT), Per1-/-, and Per2-/- mice were transduced with the BAM genes and the cells were harvested on day 0, 2, and 4 after transduction for RNA-seq