The relative activity of selected TCA cycle and ETC enzymes in the presence of Al.

All activities were expressed as percent of control.a: double bonds were measured at 220 nm. 100% corresponds to 124±10 nmol x min−1 mg protein−1 for ACN and 3.0±0.2 nmol x min−1 mg protein−1 for FUM.b: α-ketoglutarate and glyoxylate levels were measured using DNPH at 450 nm. 100% corresponds to 0.134±0.033 µmol x min−1 mg protein−1 for NAD-ICDH, 0.034±0.013 µmol x min−1 mg protein−1 for KGDH, 0.011±0.002 µmol x min−1 mg protein−1 for ICL, and 0.120±0.04 µmol x min−1 mg protein−1for MS.c: activity monitored at 600 nm using DCIP. 100% corresponds to 51±4.1 nmol x min−1 mg protein−1.d: Activity was assessed by quantifying BN-gel activity bands using SCION imaging. 100% corresponds to 5528 arbitrary units for complex I and 20392 arbitrary units for complex IV.