Rats that learn to vocalize for food reward emit longer and louder appetitive calls and fewer short aversive calls; Wardak et al.

Raw data from rats’ training to vocalize in 50-kHz ultrasonic calls (50-kHz ultrasonic vocalizations, USV) for food reward in operant conditioning. Rats were trained in six different ways (protocols 1-6), percent of rewards obtained and sessions’ duration was measured (Fig. S1). Also, percent of successful nosepokes and session duration were measured in the control experiment (Fig. S1). Based on USV-training sessions, the rats were divided into potential learners (20%) and non-learners; and into further sub-groups. Measured were: percent of rewards, call rate, session duration (Fig. 3); percent of rewarded USV in all USV, and in all 50-kHz calls (Fig. 4); duration and amplitude of 50-kHz USV (Fig. 5); and percentage of short 22-kHz USV in all USV (Fig. 6).