Single-cell transcriptomics revealed e-cigarettes-induced vascular remodeling by enhancing Tcf21 expression

Electronic cigarettes (e-cigs) are battery-powered new tobacco products attracting wide attention due to their potential in promoting smoking cessation and uncertainty in long-term health effects. According to the scientific statement from the American Heart Association (AHA), e-cig use may be a potential threat to the cardiopulmonary system and populational studies suggested that e-cig aerosol could enhance arterial stiffness Laboratory evidence suggested that e-cigs could accelerate atherosclerosis and atherosclerosis or aortic aneurysm in mice, while the exact mechanisms of e-cig-related vascular effects remained largely unknown. Here, we investigate the sub-chronic effects of e-cig aerosol on vascular structure, function, and changes in single-cell transcriptome in mice. C57BL/6J male mice aged 8 weeks inhaled filtered air (as control) or e-cig (RELX, containing glycerin, propylene glycol, spices, and 3% nicotine) 1 h per day in the exposure chamber for consecutive 90 days. The whole aorta samples collected from mice were used for single-cell transcriptome. *************************************************************** Submitter states that missing raw files are due to file loss. ***************************************************************