mRNA expression measured by qRT-PCR
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The raw data of mRNA expression of mouse liver genes - cytochromes P450 (Cyp1a2; 2a5; 2b10; 2c29; 2c38; 2d22; 2e1; 3a11; 3a13); aryl hydrocarbon receptor (Ahr); 3-hydroxy-3-methylglytaryl-3-CoA synthase 2 (Hmgcs2); 3-hydroxy-3-methylglutaryl-CoA-lyase (Hmgcl); and housekeeping gene - hypoxanthine guanine phosphoribosyl transferase. The real-time qPCR was performed on the LightCycler 1536 Instrument (Roche) using commercial TaqMan Gene primers (Thermo Fisher Scientific, Waltham, MA, USA). The 1536-well plates were pipetted using the Echo Liquid Handler (Labcyte, Dublin, Ireland). The mRNA expressions were calculated using the 2(-ΔΔC(T)) method.
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Zenodo创建时间：
2025-10-02
