HA level in 3xTg-AD mice.

Note: A: Vaccine treatment was started at 12 months. Three months later, urine was collected an entire day and HA level was measured. 3xTg-AD male mice were all homozygous. HA level with vaccine treatment decreased with time, indicating that vaccine efficacy became stronger with time.
Note: For reference, HA level in 12-month-old in 3xTg-AD male mice were observed.
Note: B: After Morris water maze test, these mice were sacrificed and their brains were immediately freezed in liquid nitrogen. Next, HA level was measured with the method described in Materials and Method.