Validation of protein expression in kidney tissue of Labeo rohita during Edwardsiella tarda infection

In this study, we examined the kidney proteome of Labeo rohita infected intraperitoneally with Edwardsiella tarda (Et). A comparative proteomic analysis of Control and Et infected kidney fwas performed. High-resolution mass spectrometry was used for gut data using label-free quantification. Discovery based proteomics data could identify a panel of differentially expressed proteins (DEPs) between Control and ET condition (Et infected group). Protein abundance changes in a few selected DEPs was then validated using selected/multiple reaction monitoring (S/MRM) approach.