Immunofluorescent staining of Choline Acetyltransferase (ChAT) in splenocytes from mPGES-1 ko or wt mice treated with LPS

Microscopy images of immunofluorescent staining of choline acetyltransferase (ChAT) in splenocytes from mPGES-1 ko (-/-) or WT (+/+) mice following 20h cultures with or without 10ng/ml LPS. (2 experiments)<br><br>Green (L5) = ChAT, Blue (A4) = DAPI, Red (N21) = CD3*<br><br>*CD3 staining gave signal in negative control samples so should be disregarded.<br><br>The protocol for staining and cell culture is described in the publication "Microsomal prostaglandin E synthase-1 gene deletion impairs neuro-immune circuitry of the cholinergic anti-inflammatory pathway in endotoxaemic mpuse spleen" published in <b>PLOS ONE 2017</b> by the authors: Priya Revathikumar, Johanna Estelius, Utsa Karmakar, Erwan LeMaître, Marina Korotkova, Per-Johan Jakobsson and Jon Lampa.