IAPV RNA-seq and BS-seq

Pollination is required for approximately 30% of food crops and honey bees (Apis mellifera) are the major managed pollinator used in agriculture. Relatively little in known on the mechanistic interactions between honey bees and their pathogens. Israel Acute Paralysis Virus (IAPV) is an important honey bee pathogen and is thought to be closely associated with colony loss. To further our understanding of its pathogenicity, we conducted BS-Seq and RNA-Seq experiments for thorough analyses on the pupae methylomic and transcriptomic profiles after IAPV infection. The host transcriptomic responses were across three different time points. These time points include an early stage of infection before the virus replicates (5 hours post-infection), and two later time points (20, and 48 hours post-infection), with the last time point including the initiation of host tissue necrosis. Two treatments are either PBS sham control or IAPV infected bee samples. Triplicate samples for each time point and each treatment are biological replicates.